Journal: Materials & Design

Article Title: Multifunctional nanofiber-based dressings in coordination with adipose-derived stem cells for accelerated burn wound healing

doi: 10.1016/j.matdes.2025.113929

Figure Lengend Snippet: Fig. 4. (A) Detection of ADSCs proliferation by ADM coating at different times. Quantification of (B) EGF and (C) FGF2 in ADSCs paracrine products. *** P < 0.001 representing a significant difference as compared with ADM (3 min) and TLWDA.

Article Snippet: Human EGF enzyme-linked immunosorbent assay (ELISA) kit and human FGF2 ELISA kit were purchased from BOSTER (Wuhan, China).

Techniques:

Journal: Neuropsychiatric Disease and Treatment

Article Title: Synapse-Related Serum and P300 Biomarkers Predict the Occurrence of Mild Cognitive Impairment in Depression

doi: 10.2147/NDT.S448312

Figure Lengend Snippet: Comparison of baseline serum indices between DWM group and DWOM group. ( A ) The comparison of serum BDNF levels. ( B ) The comparison of serum FGF2 levels. ( C ) The comparison of serum FGF7 levels. ( D ) The comparison of serum FGF22 levels.

Article Snippet: The baseline serum levels of BDNF (Proteintech, USA, Cat No. KE00096), FGF2 (Proteintech, USA, Cat No. KE00129), FGF7 (Abcam, Britain, Cat No. ab314148) and FGF22 (CUSABIO, China, Cat No. CSB-EL008628HU) in patients were measured using ELISA kits according to the manufacturer’s instructions.

Techniques: Comparison

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Morphology of bone marrow progenitor cells 48 h after infection with Ad-BMP2-bFGF-GFP at a multiplicity of infection (MOI) value of 5000. A , Image under light microscope. B , Image under fluorescence microscope showing the expression of green fluorescent protein-positive cells. Bar = 50 μm.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Infection, Light Microscopy, Fluorescence, Microscopy, Expressing

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Infection efficiency of Ad-BMP2-bFGF in bone marrow progenitor cells. The infection efficiency was calculated by the number of green fluorescent protein-positive cells/total number of nuclei stained with DAPI x100 (%) (n=3). MOI: multiplicity of infection.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Infection, Staining

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Expression of bone morphogenetic protein 2 (BMP-2) and basic fibroblast growth factor (bFGF) in bone marrow progenitor cells (BMPCs). A , ELISA assay of BMP2 level secreted by BMPCs at different days after infection. B , ELISA assay of bFGF level secreted by BMPCs at different days after infection. Data are reported as means±SD (n=3). *P<0.05 vs control (ANOVA).

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Infection, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Alkaline phosphatase (ALP) activity in bone marrow progenitor cells (BMPCs). A , ALP activity detected in BMPCs at different days after infection. *P<0.05 vs control. # P<0.05 vs Ad-BMP2-infected BMPCs. B , ALP staining of BMPCs 21 days after infection with Ad-BMP2-bFGF. Many brown-black particles appeared in the cytoplasm, and cells were stained blue. Bar = 100 μm.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Activity Assay, Infection, Control, Staining

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Kossa staining of bone marrow progenitor cells 21 days after infection with Ad-BMP2-bFGF or Ad-BMP2. Mineralized calcium deposits were stained as dark nodules. Bar = 400 μm.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Staining, Infection