Journal: Materials & Design

Article Title: Multifunctional nanofiber-based dressings in coordination with adipose-derived stem cells for accelerated burn wound healing

doi: 10.1016/j.matdes.2025.113929

Figure Lengend Snippet: Fig. 4. (A) Detection of ADSCs proliferation by ADM coating at different times. Quantification of (B) EGF and (C) FGF2 in ADSCs paracrine products. *** P < 0.001 representing a significant difference as compared with ADM (3 min) and TLWDA.

Article Snippet: Human EGF enzyme-linked immunosorbent assay (ELISA) kit and human FGF2 ELISA kit were purchased from BOSTER (Wuhan, China).

Techniques:

Journal: Regenerative Biomaterials

Article Title: Specific bFGF targeting of KIM-1 in ischemic kidneys protects against renal ischemia-reperfusion injury in rats

doi: 10.1093/rb/rbac029

Figure Lengend Snippet: Purification, bioactivity and targeting capacity of KIT-bFGF. ( A ) Protein structure prediction of recombinant KIT-bFGF and native bFGF. ( B ) Purified KIT-bFGF and native bFGF were detected on the SDS-PAGE gel. ( C ) Purified KIT-bFGF and native bFGF were detected by western blot. ( D ) The bioactivity of KIT-bFGF and native bFGF was detected by MTT assay through the promotion of HSFs proliferation. ( E ) The expression of KIM-1 in HK-2 cells after I/R injury in vitro by western blot. ( F ) The expression of KIM-1 in ischemic kidneys after I/R injury in vivo by western blot. ( G ) Fluorescent observation of targeting capacity of the KIT-bFGF and bFGF proteins labeled with DyLight-747 dye after HK-2 I/R injury for 12 h. The scale bar = 50 μm. ( H ) Cell survival was detected by MTT after KIT-bFGF and bFGF treatment in HK-2 cells after I/R injury in vitro. * P < 0.05.

Article Snippet: And then, extracted proteins and serum were analyzed using a human bFGF ELISA kit (Boster, Wuhan, China) according to the protocol.

Techniques: Purification, Recombinant, SDS Page, Western Blot, MTT Assay, Expressing, In Vitro, In Vivo, Labeling

Journal: Regenerative Biomaterials

Article Title: Specific bFGF targeting of KIM-1 in ischemic kidneys protects against renal ischemia-reperfusion injury in rats

doi: 10.1093/rb/rbac029

Figure Lengend Snippet: KIT-bFGF could target to the ischemic kidney and retain in the kidney after renal I/R injury. ( A ) Quantitative ELISA assay for bFGF in kidney at 6 and 24 h postinjection. At 6 h postinjection, KIT-bFGF = 24.507 ± 2.002 μg/g, bFGF = 13.523 ± 3.704 μg/g and PBS = 8.208 ± 1.562 μg/g. At 24 h postinjection, KIT-bFGF = 29.696 ± 0.682 μg/g, bFGF = 5.618 ± 0.266 μg/g and PBS = 5.572 ± 0.746 μg/g. Data are presented as mean ± SD. N = 6, ** P < 0.01, *** P < 0.001, **** P < 0.0001. ( B ) Quantitative ELISA assay for bFGF in serum. At 6 h postinjection, KIT-bFGF = 9.407 ± 1.849 μg/ml, bFGF = 8.214 ± 0.974 μg/ml and PBS = 12.399 ± 4.141 μg/ml. At 24 h postinjection, KIT-bFGF = 5.315 ± 0.292 μg/ml, bFGF = 5.554 ± 0.410 μg/ml and PBS = 5.873 ± 0.464 μg/ml. Data are presented as mean ± SD. N = 6. ( C , a–d) Animals' imaging for fluorescence DyLight-747 dye labeled KIT-bFGF and bFGF at 6 and 24 h postinjection in vivo by fluorescence imaging system. Ex/Em = 748/771. (e–h) Fluorescence distribution in kidneys after 6 and 24 h administration. Scale bar = 50 μm. N = 6. ( D, E ) Immunofluorescence staining for bFGF (green) and KIM-1 (red) colocalization (yellow) at 6 and 24 h postadministration. Scale bar = 20 μm. Each slice representative of ×400 original magnification. N = 6.

Article Snippet: And then, extracted proteins and serum were analyzed using a human bFGF ELISA kit (Boster, Wuhan, China) according to the protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Imaging, Fluorescence, Labeling, In Vivo, Immunofluorescence, Staining

Journal: Regenerative Biomaterials

Article Title: Specific bFGF targeting of KIM-1 in ischemic kidneys protects against renal ischemia-reperfusion injury in rats

doi: 10.1093/rb/rbac029

Figure Lengend Snippet: KIT-bFGF improved the recovery of renal function after acute renal I/R injury. ( A ) Summary of Scr data for renal function after administration. * P < 0.05. ** P < 0.01. Data are presented as mean ± SD ( B–E ) Histogram of Scr assay for renal function after I/R injury and at 24, 72 h, 2 and 4-week postadministration. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: And then, extracted proteins and serum were analyzed using a human bFGF ELISA kit (Boster, Wuhan, China) according to the protocol.

Techniques:

Journal: Regenerative Biomaterials

Article Title: Specific bFGF targeting of KIM-1 in ischemic kidneys protects against renal ischemia-reperfusion injury in rats

doi: 10.1093/rb/rbac029

Figure Lengend Snippet: KIT-bFGF protected kidney against acute renal I/R injury. ( A–D ) Histopathological staining (H&E) for kidney pathological injury observation. Each slice representative of ×200 original magnification. Scale bar = 50 μm. ( E–H ) Quantitative analysis of renal tubule injury based on H&E staining. All data are expressed as mean ± SD. **** P < 0.0001. N = 8.

Article Snippet: And then, extracted proteins and serum were analyzed using a human bFGF ELISA kit (Boster, Wuhan, China) according to the protocol.

Techniques: Staining

Journal: Regenerative Biomaterials

Article Title: Specific bFGF targeting of KIM-1 in ischemic kidneys protects against renal ischemia-reperfusion injury in rats

doi: 10.1093/rb/rbac029

Figure Lengend Snippet: KIT-bFGF attenuated the renal fibrosis after acute renal I/R injury. ( A, B ) Blocked renal interstitial volume (blue staining and octothorpe) assay by using Masson’s trichrome staining. ×100 original magnification. Scale bar = 100 μm. ( C, D ) Quantitative analysis of the area of renal interstitial fibrosis in obstructed kidneys. All data are expressed as mean ± SD. **** P < 0.0001. N = 8.

Article Snippet: And then, extracted proteins and serum were analyzed using a human bFGF ELISA kit (Boster, Wuhan, China) according to the protocol.

Techniques: Staining

Journal: Regenerative Biomaterials

Article Title: Specific bFGF targeting of KIM-1 in ischemic kidneys protects against renal ischemia-reperfusion injury in rats

doi: 10.1093/rb/rbac029

Figure Lengend Snippet: KIT-bFGF inhabited the apoptosis through the regulation of proapoptotic proteins. ( A–D ) TUNEL staining for apoptosis cells assessment (green). ×200 original magnification, scale bar = 50 μm. ( E–H ) Quantitative of kidney TUNEL-positive cells. All data are expressed as mean ± SD. *** P < 0.001, **** P < 0.0001. N = 8.

Article Snippet: And then, extracted proteins and serum were analyzed using a human bFGF ELISA kit (Boster, Wuhan, China) according to the protocol.

Techniques: TUNEL Assay, Staining

Journal: Regenerative Biomaterials

Article Title: Specific bFGF targeting of KIM-1 in ischemic kidneys protects against renal ischemia-reperfusion injury in rats

doi: 10.1093/rb/rbac029

Figure Lengend Snippet: KIT-bFGF inhabited the apoptosis through the regulation of proapoptotic proteins. ( A–D ) IHC staining for cleaved-Caspase3 after I/R injury and administration. ×200 original magnification, scale bar = 50 μm. ( E–H ) Quantitative of IHC staining for cleaved caspase-3. All data are expressed as mean ± SD. ** P < 0.01, **** P < 0.0001. N = 8. (E) At 24-h postadministration: NORMAL = 0.40 ± 0.52, KIT-bFGF = 1.14 ± 0.69, bFGF = 1.63 ± 0.74, PBS= 2.29 ± 0.49. (F) At 72-h postadministration: KIT-bFGF = 0.75 ± 0.46, bFGF = 1.88 ± 0.35, PBS= 3.13 ± 1.36. (G) At 2-week postadministration: KIT-bFGF = 0.50 ± 0.53, bFGF = 1.63 ± 0.52, PBS= 5.25 ± 1.49. (H) At 4-week postadministration: KIT-bFGF = 0.50 ± 0.53, bFGF = 3.00 ± 1.87, PBS= 4.00 ± 1.58. ( I–L ) Western blot assayed the expression of cleaved Caspase-3, Bcl-2, ERK1/2, phospho-ERK1/2, Akt and phospho-Akt at 24, 72 h, 2-and 4-week postadministration. β-actin and GAPDH were used as a control.

Article Snippet: And then, extracted proteins and serum were analyzed using a human bFGF ELISA kit (Boster, Wuhan, China) according to the protocol.

Techniques: Immunohistochemistry, Western Blot, Expressing, Control